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One kind is applied to the syringe pump dosing methods and processes

发布日期:2020-01-07 13:12 Document serial number: 19641811 Release date: 2020-01-07 13:12
One kind is applied to the syringe pump dosing methods and processes

The invention is a liquid adding method applied to a syringe pump, and belongs to the technical field of measurement and testing.



Background technique:

In all kinds of chemical, biological and other experiments, most of the experimental samples need to be pre-processed, and the development of sample pre-processing technology has slowly restricted the development of analytical chemistry. With the increasing nature of the samples and the complexity of the steps, the test cycle has become longer. At the same time, the current sample pre-treatment device mainly uses a syringe pump to dispense the liquid. The reagent will remain on the inner wall of the pipeline when the syringe pump dispenses the liquid, which will eventually lead to deviations in the results.

In addition, different types of reagents need to be added during the pretreatment of large quantities of samples. Each injection pump of the reagent needs to rinse the inlet pipe, outlet pipe, and syringe of the syringe pump, which will increase the workload of the syringe pump. , Resulting in reduced efficiency; and wasted reagents, resulting in waste of resources and increased costs.



Technical realization elements:

In order to solve the above problems, the present invention proposes a liquid adding method applied to a syringe pump, which can solve the problem of liquid adding errors caused by residual liquid in the liquid outlet pipe while pre-processing large quantities of samples, and guarantee high efficiency. Under the circumstances, the problem of preventing cross-contamination of two or more reagents and saving reagents is avoided.

The object of the present invention is achieved by the following technical solutions:

The liquid adding method applied to a syringe pump is characterized in that the steps of the method are as follows:

Step one, preparation

The vessel used in the method includes a mother liquid bottle 1, a sample bottle 2, a waste liquid cup 3, a pure water reagent bottle 4 and a syringe pump 5. The syringe pump 5 is equipped with a y-type valve 6, and a y-type valve 24 is connected to the liquid inlet pipe. 8 and the discharge tube 9 and the injection needle tube 15;

Step two, pure water cleaning

The liquid inlet tube 8 is placed in the pure water reagent bottle 4 and the liquid outlet tube 9 is placed above the waste liquid cup 3. The injection pump 5 extracts pure water from the pure water reagent bottle 4 through the liquid inlet tube 8 and sequentially switches the y type The liquid outlet direction of the valve 24 cleans the liquid inlet pipe 8, the liquid outlet pipe 9, and the injection needle tube 15;

Step three: air cleaning

The liquid inlet tube 8 is placed in the air or an empty reagent bottle. The syringe pump 5 draws air through the liquid inlet tube 8 and sequentially switches the air inlet tube 8, the liquid outlet tube 9 and the injection needle tube 15 by switching the air outlet direction of the y valve 24 Residual pure water in the drainage;

Step 4.Add a reagent

Insert the liquid outlet tube 9 into the mother liquid bottle 1 and use the syringe pump 5 to extract the required amount of reagent. Then insert the liquid outlet tube 9 into the sample bottle 2 and inject the reagent in the liquid outlet tube 9 into the sample bottle 2. The syringe pump 5 draws air through the liquid inlet pipe 8 and injects the air in the liquid inlet pipe 8 into the sample bottle 2 through the liquid outlet pipe 9 to discharge the reagent remaining in the liquid outlet pipe 9;

Step 5.Add other reagents

Repeat the operations of steps 2 and 3 and dry the outer wall of the liquid tube 9, repeat the operation of step 4 to complete the addition of other reagents;

Step 6. End and clean

Repeat steps 2 and 3, and then place the liquid outlet tube 9 above the waste liquid cup 3.

In one implementation, the operation of step four is: after using the syringe pump 5 to extract air through the liquid outlet pipe 9, the required amount of reagent is drawn from the mother liquid bottle 1, and then the air and reagent in the liquid outlet pipe 9 are injected into the sample Inside the bottle 2.

In one implementation, a reagent rinse step is added between steps three and four. The step is that the syringe pump 5 extracts the reagent from the mother liquid bottle 1 through the liquid discharge pipe 9 and then discharges the reagent in the liquid discharge pipe 9 to waste. In the liquid cup 3, step 3 is repeated to discharge the remaining reagents in the liquid discharge pipe 9 into the waste liquid cup 3.

In one implementation, the syringe needle 15 on the syringe pump 5 is replaced to meet the needs of different capacities, and the capacity ranges from 100 microliters to 25 milliliters.

In one implementation, the amount of air extracted in step four cannot be less than 600 microliters.

6. The method for adding liquid to a syringe pump according to claim 2, characterized in that the amount of extracted air cannot be less than 200 microliters.

In one implementation, the number of times of pure water cleaning is 1 to 3 times.

In one implementation, the number of air cleaning is 2 to 3 times.

In one implementation, the number of rinses of the reagent is 1 to 3 times.

BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1 Schematic diagram of the syringe pump

Fig. 2 Schematic diagram of adding liquid to the syringe pump

Figure 3 Schematic diagram of the structure of the automatic device for adding liquid to the syringe pump

detailed description

The technical solution of the present invention will be further detailed below with reference to the accompanying drawings and embodiments:

As shown in FIG. 3, the automatic device for adding liquid to the syringe pump in this embodiment includes a syringe pump 5, a drying device 6, a liquid inlet tube 8, a liquid outlet tube 9, an injection needle tube 15, a three-dimensional motion platform, a c-axis 11, and a working device. A platform 17, a peristaltic pump 22, and a control system 12, the three-dimensional motion platform includes an x-axis 13, a y-axis, and a z-axis. The liquid outlet pipe 9 is fixed on the x-axis 13, and the liquid inlet pipe 8 is fixed on c. On the axis 11, the control system 12 controls the three-dimensional motion platform 10 to drive the liquid outlet pipe 9 to move up, down, and to the left and right, and the control system 12 controls the c axis 11 to drive the liquid inlet pipe 8 to move up and down. The working platform 17 described in FIG. 2 is a 4 * 5 platform. The number and position of mother liquid bottles and sample bottles of different sizes can be arranged according to actual needs, and the waste liquid cup 3 occupies one position; the wipe dry A water-absorbing material such as a sponge is fixed inside the top ends of the two clamping arms 20 of the device 6.

As shown in Figures 1 and 2, the method of adding liquid to a syringe pump in this implementation is as follows:

1) Prepare, place mother liquid bottle 1, sample bottle 2 and waste liquid cup 3 on work platform 17, pure water reagent bottle 4 on the side of the injection pump 5, and clean water reagent bottle 23 on the side of the peristaltic pump 22; A y-type valve 24 is installed on the syringe pump 5, and the liquid inlet pipe 8 and the liquid outlet pipe 9 and the injection needle pipe 15 are respectively connected through the y-type valve 24;

2) The outer wall is scrubbed, the x-axis 13 is controlled by the control system 12 to drive the liquid outlet pipe 9 to move above the waste liquid bottle 3, and the peristaltic pump 22 draws clean water from the clean water reagent bottle 23 to clean the The outer wall of the liquid outlet pipe 9, and then the control system 12 controls the opening and closing of the two clamping arms 20 of the drying device 6 to wipe the clean water of the outer wall of the liquid outlet pipe 9 with a sponge;

3) Pure water cleaning, the control system 12 controls the x-axis 13 to drive the liquid outlet pipe 9 to move above the waste liquid cup 3, and the control system 12 controls the c-axis 11 to drive the liquid inlet A tube 8 is inserted into the pure water reagent bottle 4, the injection pump 5 draws pure water through the liquid inlet tube 8, switches the y-shaped valve 24 to the liquid outlet end 9, and the injection needle tube 15 passes through The liquid outlet pipe 9 discharges pure water into the waste liquid cup 3, and cleans the liquid inlet pipe 8, the liquid outlet pipe 9, and the injection needle tube 15;

4) Air cleaning, switching the y-shaped valve 24 to the liquid inlet end 8 and driving the liquid inlet tube 8 upward by the c-axis 11 so that the liquid inlet tube 8 leaves the pure water reagent bottle 4, the The syringe pump 5 draws air through the liquid inlet pipe 8 and switches the y-shaped valve 24 to the liquid outlet end 9. The syringe pump 5 discharges air through the liquid outlet pipe 9 and the liquid outlet pipe 9 Residual pure water is drained;

5) Add a reagent, method one: the y-shaped valve 24 stays at the end of the outlet pipe 9 and the x-axis 13 drives the outlet pipe 9 to move into the mother liquid bottle 1, the injection pump 5 Withdraw a certain amount of required reagents through the liquid discharge tube (9), and then drive the liquid discharge tube 9 into the sample bottle (2) through the x-axis 13 to remove the reagents in the liquid discharge tube 9 Inject into the sample bottle 2; switch the y-shaped valve 24 to the end of the liquid inlet pipe 8, and then the injection pump 5 draws air through the liquid inlet 8 and switch the y-shaped valve 24 to the liquid outlet pipe At 9 ends, the syringe pump 5 discharges air through the liquid outlet pipe 9 and discharges the remaining reagents in the liquid outlet pipe 9 to the sample bottle 2;

Method two: the y-shaped valve 24 stays at the end of the liquid outlet pipe 9, and the liquid pipe 9 is driven into the mother liquid bottle 1 by the x-axis 13, and the injection pump 5 passes through the liquid outlet pipe 9 extract a certain amount of air, then insert the x-axis 13 to drive the liquid outlet tube (9) into the mother liquid bottle 1 to extract the required amount of reagent, and then the x-axis 13 drives the liquid outlet tube 9 to In the sample bottle 2, the syringe pump 5 evacuates the syringe needle 15 through the syringe pump 5 to inject air and reagents in the liquid discharge tube 9 into the sample bottle 2,

6) Add multiple reagents. After one reagent is added, repeat steps 2), 3) 4) and 5) until all reagents are added.

7) End and clean, repeat steps 2), 3) and 4), the liquid outlet pipe (9) moves to and stays above the waste liquid cup (3).

A reagent rinsing step may be added between the steps 3) and 4), the y-shaped valve 24 is switched to the end of the liquid outlet pipe 9, the liquid outlet pipe 9 is moved into the mother liquid bottle 1 and extracted For a certain amount of reagent, move the liquid discharge tube 9 above the waste liquid cup 3, and discharge the reagent in the liquid discharge tube 9 into the waste liquid cup 3 through the syringe pump 5, and then repeat step 3 ), The reagent remaining in the liquid discharge tube 9 is discharged into the waste liquid cup 3, and the number of times of reagent rinsing is 1 to 3 times. The rinse step is used when the cleaning requirements are high and the accuracy of the data requirements is extremely high. The rinse step only rinses the outlet pipe, which saves reagents and prevents cross-contamination of two or more reagents.

In step 2), the injection pump 5 extracts pure water to clean the liquid inlet pipe 8, liquid outlet pipe 9, and the injection needle pipe 15 to effectively avoid cross-infection between reagents. The air cleaning in step 3) can effectively remove Residual pure water is drained to avoid adding error. In the first method in step 4), the liquid outlet pipe 9 first extracts and discharges the reagent, and the liquid inlet pipe 8 extracts air, and the air is discharged by using the exhaust air. The remaining reagents in the liquid pipe 9 are drained; the second method is that the liquid pipe 9 first extracts air and then extracts the required reagents. When adding liquid, the liquid pipe 9 first adds the required reagents and then discharges air, which helps The reagents are all discharged to avoid a small amount of reagents remaining on the inner wall of the liquid outlet pipe 9.

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